Repository series · ncbi-prjna748027
RNA sequencing of Sparidentex hasta larvae exposed to water extractable fraction of Kuwait crude oil
The present study was performed to understand the effects of petroleum hydrocarbons on early life stages of development of marine fish species. The study specifically focuses on determining the genotoxic effects on sobaity sea bream (Sparidentex hasta) fish larvae exposed to Kuwait crude oil (KCO). Newly hatched sobaity sea bream larvae were exposed to water-accommodated fractions (WAF) of KCO. The total RNA was isolated at different time intervals from control and WAF treated larvae using Trizol reagent. Paired-end based RNA sequencing was performed. Differential expression analysis was performed to study the changes in gene expression in control and WAF exposed sobaity sea bream larvae at 3, 6, 24, 48, 72 or 96 h time points.
The accession and its GCC connection are verified. Registration alone does not establish that the broader research programme remains active.
01 / Project overview
What the record establishes.
- Geographic scope
- Kuwait connection indexed in BioProject metadata
- Project type
- Repository project
- Research domain
- Environmental and microbial genomics
- Years
- 2021–
- Lifecycle status
- repository_recorded
- Status basis
- Registered in NCBI BioProject on 2021/07/19; operational lifecycle is not asserted.
- Status evidence date
- 2021-07-19
- Scale
- 1 BioProject accession grouped by matching submitter, date, data type and narrative.
02 / Organizations and population
Who and what the project connects.
- Lead organizations
- Kuwait Institute for Scientific Research (KISR)
- Partner organizations
- Not stated
- Organism / population
- Not stated
03 / Data and access
What exists and how it can be reached.
Data types
- Raw sequence reads
- Sequencing
- Genome
Data access
Public repository metadata with linked data where supplied by the submitter
Identifiers
- BioProject
PRJNA748027
04 / Evidence and provenance
Why the record is included.
Inclusion basis
Exact country-name match in authoritative NCBI BioProject metadata; repeated submissions are grouped into one Atlas series.
Editorial note
Repository verification confirms the accession and regional connection, not whether the broader research programme remains active.
Sources
- primary record source Verified 2026-08-15
Release v0.2.0